ABSTRACT
Objective: To study the triterpene acids from the roots of Symplocos laurina. Methods: The constituents were isolated by repeated chromatography with silica gel, Sephadex LH-20, ODS columns, and semi-preparing liquid chromatography. The structures were elucidated by sepectroscopic anaylsis. Results: Ten compounds were isolated from 95% ethanol extract of the roots of S. laurina and their structures were identified as: 19α-hydroxy-4-oxo-3,24-dinor-2,4-secoolean-12-en-2,28-dioic acid (1), negundonorin A (2), 2α,3β,19α,23-tetrahydroxyurs-12-en-28-oic acid (3), 2α,3β,19α,23-tetrahydroxylean-12-en-28-oic acid (4), 2α,3β,23-trihydroxyurs- 12-en-28-oic acid (5), 2α,3β,23-trihydroxylean-12-en-28-oic acid (6), 2α,3α,19α,23-tetrahydroxyurs-12-en-28-oic acid (7), 1α,3β,19α,23-tetrahydroxyurs-12-en-28-oic acid (8), 3β,19α,23-trihydroxyurs-12-en-28-oic acid (9), and 2-oxo-3β,19α,23- trihydroxyurs-12-en-28-oic acid (10). Conclusion: All the compounds are isolated from this plant for the first time. In addition to compounds 3 and 7, the other compounds are isolated from the plants of Symplocos Jacq for the first time.